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| Status |
Public on Oct 03, 2017 |
| Title |
Generation of mature lung alveolar epithelial cells from human pluripotent stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We perform time-series global transcriptomic profiling of a directed differentiation protocol for generating alveolar epithelial type II cells (AEC2s) from pluripotent stem cells (PSCs). We analyzed 3 different timepoints in the RUES2 differentiation: 1) Day 0 undifferentiated cells, 2) Day 15 lung progenitors highly enriched in NKX2-1+ cells by CD47hi/CD26lo sorting, and 3) the outgrowth of these purified progenitors in 3D culture sorted again on Day 35 based on SFTPCtdTomato+ and SFTPCtdTomato- gating. For comparison to primary cells, we simultaneously sequenced RNA from purified primary fetal (21 week gestation) distal alveolar epithelial progenitors and purified adult human (HTII-280 sorted) AEC2s. In order to evaluate the effect of cell culture on primary fetal alveolar cells, parallel aliquots of the fetal cells were also exposed to 4 days of culture in DCI media. We find that AEC2 maturation involves downregulation of Wnt signaling activity, and that the highest differentially expressed transcripts in iPSC-derived AEC2s encode genes associated with lamellar body and surfactant biogenesis.
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| Overall design |
Analysis of global transcriptomes of PSCs and their differentiated progeny (primordial lung progenitors and alveolar cells) compared to primary fetal (21 week) and adult alveolar epithelial type 2 cells in triplicate
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| |
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| Contributor(s) |
Jacob A, Kotton DN |
| Citation(s) |
28965766 |
| Submission date |
Mar 15, 2017 |
| Last update date |
May 24, 2019 |
| Contact name |
Anjali Jacob |
| E-mail(s) |
ajacob@Bu.edu
|
| Organization name |
Boston University School of Medicine
|
| Department |
Center for Regenerative Medicine
|
| Lab |
Kotton Lab
|
| Street address |
670 Albany St., 2nd Floor, CReM-232
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA379280 |
| SRA |
SRP101943 |
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