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Series GSE94802 Query DataSets for GSE94802
Status Public on Mar 16, 2017
Title Retinoic acid signaling is dispensable for somatic development and function of the developing ovary
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Retinoic acid (RA) is a potent inducer of cell differentiation and plays an essential role in sex-specific germ cell development in the mammalian gonad. RA is essential for male gametogenesis and hence fertility. However, RA can also disrupt sexual cell fate in somatic cells of the testis, promoting transdifferentiation of male Sertoli cells to female granulosa-like cells when the male sexual regulator Dmrt1 is absent. The feminizing ability of RA in the somatic testis suggests that RA might normally play a role in somatic cell differentiation or cell fate maintenance in the ovary. To test for this possibility we disrupted RA signaling in somatic cells of the early fetal ovary using three genetic strategies and one pharmaceutical approach. We found that deleting all three RA receptors (RARs) in the XX somatic gonad at the time of sex determination did not significantly affect ovarian differentiation, follicle development, or female fertility. Transcriptome analysis of adult triple mutant ovaries revealed remarkably little effect on gene expression in the absence of somatic RAR function. Likewise, deletion of three RA synthesis enzymes (Aldha1-3) at the time of sex determination did not masculinize the ovary. A dominant-negative RAR transgene altered granulosa cell proliferation, likely due to interference with a non-RA signaling pathway, but did not affect granulosa cell specification or fertility. Finally, culture of fetal XX gonads with an RAR antagonist blocked germ cell meiotic initiation but did not disrupt sex-biased gene expression. We conclude that RA signaling, although crucial in the ovary for meiotic initiation, is not required for granulosa cell specification, differentiation, or reproductive function.
Overall design Ovaries from six week old mice with five replicates in each of two genotypes were analyzed by RNA-Seq
Web link
Contributor(s) Minkina A, Gearhart MD, Zarkower D
Citation(s) 28274610
Submission date Feb 11, 2017
Last update date May 15, 2019
Contact name Micah Gearhart
Organization name University of Minnesota
Department Genetics, Cell Biology and Development
Street address 6-160 Jackson Hall, 321 Church St.
City Minneapolis
State/province MN
ZIP/Postal code 55455
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (10)
GSM2483396 Dmrt1_het_Rar_mutant_ovary_rep1_7558_S28
GSM2483397 Control_ovary_rep1_7707_S27
GSM2483398 Dmrt1_het_Rar_mutant_ovary_rep2_7713_S29
BioProject PRJNA374406
SRA SRP099303

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Supplementary file Size Download File type/resource
GSE94802_Minkina_etal_normalized_FPKM.csv.gz 782.0 Kb (ftp)(http) CSV
GSE94802_Minkina_etal_raw_counts.csv.gz 560.6 Kb (ftp)(http) CSV
GSE94802_RAW.tar 1.4 Gb (http)(custom) TAR (of BIGWIG)
GSE94802_Supplementary_Table_1.csv.gz 11.7 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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