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Series GSE78688 Query DataSets for GSE78688
Status Public on Nov 02, 2016
Title Activity-dependent transcriptional changes in human neurons
Organisms Homo sapiens; Mus musculus; Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary We used genome-wide sequencing methods to identify activity-regulated gene expression and regulatory elements in human neurons. Sensory stimuli drive the maturation and function of the mammalian nervous system in part through the activation of gene expression networks that regulate synapse development and plasticity. These programs of gene transcription have primarily been studied in mice, and it is not known if there are neuronal activity-regulated genes that control features of brain development and function that are unique to humans. In this study, we used genome-wide sequencing methods to identify activity-regulated gene expression and regulatory elements in human neurons.
 
Overall design Total RNA-seq data was obtained from primary human, mouse, and rat neuronal cultures that are depolarized for 0, 1, and 6 hours, as well as ChIP-seq data using H3K4me1, H3K4me3, and H3K27ac antibodies.
 
Contributor(s) Ataman B, Boulting GL, Harmin DA, Greenberg ME
Citation(s) 27830782
Submission date Feb 25, 2016
Last update date Mar 19, 2019
Contact name Bulent Ataman
Organization name Harvard Medical School
Department Neurobiology
Lab Michael E. Greenberg
Street address 220 Longwood Ave. GB405
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (4)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (43)
GSM2072621 Human rep 1 neurons 0hr post-KCl stim
GSM2072622 Human rep 1 neurons 1hr post-KCl stim
GSM2072623 Human rep 1 neurons 6hr post-KCl stim
Relations
BioProject PRJNA313218
SRA SRP070845

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Supplementary file Size Download File type/resource
GSE78688_RAW.tar 10.1 Gb (http)(custom) TAR (of BW)
Raw data are available in SRA
Processed data provided as supplementary file

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