|
| Status |
Public on Feb 16, 2022 |
| Title |
Collagen10a1-Cre;Rosa26-tdTomato Bone marrow colony forming units |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To assess if descendants of the hypertrophic chondrocytes behaved as skeletal stem and progenitor cells, we performed colony unit formation. We observed a near equal proportion of colonies derived from hypertrophic chondrocytes (tomato+) and derived from other sources (likely periosteum). We performed bulk RNA-sequencing on 3 colonies from each origin to compare the gene profiles and potential differentiation capacity or niche support.
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| |
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| Overall design |
Col10a1-Cre;R26-tdTomato, Tomato + and Tomato - colonies 10 days post plating
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| |
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| Contributor(s) |
Long JT, Leinroth A, Liao Y, Ren Y, Mirando AJ, Nguyen T, Guo W, Sharma D, Wu C, Cheah KS, Karner CM, Hilton MJ, Rouse D |
| Citation(s) |
35179487 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AR071722 |
Notch Signaling in Endochondral Bone Development |
DUKE UNIVERSITY |
Matthew J. Hilton |
| R01 AR063071 |
Notch Signaling in Joint Cartilage Maintenance and Arthritis |
DUKE UNIVERSITY |
Matthew J. Hilton |
|
| |
| Submission date |
Jun 30, 2021 |
| Last update date |
Mar 09, 2022 |
| Contact name |
Matthew J. Hilton |
| E-mail(s) |
matthew.hilton@duke.edu
|
| Phone |
9196139761
|
| Organization name |
DUKE UNIVERSITY SCHOOL OF MEDICINE
|
| Street address |
450 Research Drive, LSRC B321C
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (6)
|
| GSM5410997 |
Col10a1-Cre;R26-tdTomato Bone marrow cells [Tomato 1] |
| GSM5410998 |
Col10a1-Cre;R26-tdTomato Bone marrow cells [Tomato 2] |
| GSM5410999 |
Col10a1-Cre;R26-tdTomato Bone marrow cells [Tomato 3] |
| GSM5411000 |
Col10a1-Cre;R26-tdTomato Bone marrow cells [NonTomato 1] |
| GSM5411001 |
Col10a1-Cre;R26-tdTomato Bone marrow cells [NonTomato 2] |
| GSM5411002 |
Col10a1-Cre;R26-tdTomato Bone marrow cells [NonTomato 3] |
|
| Relations |
| BioProject |
PRJNA742567 |
| SRA |
SRP326237 |
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