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Series GSE114077 Query DataSets for GSE114077
Status Public on Apr 01, 2019
Title Proteotoxicity from aberrant ribosome biogenesis compromises cell fitness
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Summary To achieve maximal growth, cells must manage a massive economy of ribosomal proteins (r-proteins) and RNAs (rRNAs) to produce thousands of ribosomes every minute. Although ribosomes are essential in all cells, natural disruptions to ribosome biogenesis lead to heterogeneous phenotypes. Here, we model these perturbations in Saccharomyces cerevisiae and show that challenges to ribosome biogenesis result in acute loss of proteostasis. Imbalances in the synthesis of r-proteins and rRNAs lead to the rapid aggregation of newly synthesized orphan r-proteins and compromise essential cellular processes, which cells alleviate by activating proteostasis genes. Exogenously bolstering the proteostasis network increases cellular fitness in the face of challenges to ribosome assembly, demonstrating the direct contribution of orphan r-proteins to cellular phenotypes. We propose that ribosome assembly is a key vulnerability of proteostasis maintenance in proliferating cells that may be compromised by diverse genetic, environmental, and xenobiotic perturbations that generate orphan r-proteins.
 
Overall design RNA-seq from yeast cells treated in the indicated condition
 
Contributor(s) Tye BW, Churchman LS
Citation(s) 30843788
Submission date May 06, 2018
Last update date Apr 02, 2019
Contact name Blake W. Tye
E-mail(s) btye@fas.harvard.edu
Organization name Harvard Medical School
Street address 77 Ave Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL19756 Illumina NextSeq 500 (Saccharomyces cerevisiae)
Samples (30)
GSM3132414 WTAID_Unt_repA
GSM3132415 WTAID_Unt_repB
GSM3132416 WTAID_Aux_repA
Relations
BioProject PRJNA464238
SRA SRP144685

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114077_150520-WT-DiaT00-RNA-RPKM-Both.txt.gz 98.0 Kb (ftp)(http) TXT
GSE114077_150520-WT-DiaT15-RNA-RPKM-Both.txt.gz 98.7 Kb (ftp)(http) TXT
GSE114077_Las1AID_Unt_Aux_DESeq.csv.gz 587.0 Kb (ftp)(http) CSV
GSE114077_Rat1AID_Unt_Aux_DESeq.csv.gz 601.2 Kb (ftp)(http) CSV
GSE114077_Rrp17AID_Unt_Aux_DESeq.csv.gz 541.9 Kb (ftp)(http) CSV
GSE114077_Rrp44AID_Unt_Aux_DESeq.csv.gz 599.5 Kb (ftp)(http) CSV
GSE114077_WTAID_Unt_Aux_DESeq.csv.gz 554.3 Kb (ftp)(http) CSV
GSE114077_WT_DMSO_Diazaborine_15min_DESeq.csv.gz 590.4 Kb (ftp)(http) CSV
GSE114077_WT_DMSO_Diazaborine_45min_DESeq.csv.gz 613.1 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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