|
| Status |
Public on Sep 15, 2006 |
| Title |
TCHW P5_m103 salt stress, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
rice crown and growing point tissue
|
| Organism |
Oryza sativa Japonica Group |
| Characteristics |
salt sensitive
|
| Treatment protocol |
salt stressed growth condition
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000.
|
| Description |
Gene expression data from unstressed crown and growing point tissue of rice
|
| Data processing |
The data were analyzed with GCOS 1.2 using Affymetrix default analysis settings and global scaling as normalization method
|
| |
|
| Submission date |
Mar 10, 2006 |
| Last update date |
Mar 10, 2006 |
| Contact name |
HARKAMAL WALIA |
| E-mail(s) |
waliah02@student.ucr.edu
|
| Phone |
951-827-3808
|
| Organization name |
University of California
|
| Street address |
Department of Botany & Plant Sciecnes
|
| City |
Riverside |
| State/province |
CA |
| ZIP/Postal code |
92521 |
| Country |
USA |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE4438 |
Expression data from rice under salinity stress |
|
