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Status |
Public on May 31, 2013 |
Title |
Ramos cell line, replicate 3 |
Sample type |
RNA |
|
|
Source name |
Human B lymphocyte cell line (Burkitt's lymphoma)
|
Organism |
Homo sapiens |
Characteristics |
cell line: Ramos
|
Treatment protocol |
No treatment has been applied.
|
Growth protocol |
Cells were grown under standard conditions.
|
Extracted molecule |
total RNA |
Extraction protocol |
The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
|
Label |
biotin
|
Label protocol |
The sense cDNA was prepared from 300 ng of total RNA using the Ambion® WT Expression Kit. The sense strand cDNA was then fragmented and biotinylated with the Affymetrix GeneChip® WT Terminal Labeling Kit (PN 900671).
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|
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Hybridization protocol |
Labeled sense cDNA was hybridized to the Affymetrix HuGene-1_0-st chip according to the the manufacturer protocols and using GeneChip® Hybridization, Wash and Stain Kit.
|
Scan protocol |
Genechips were scanned with the Affymetrix GeneChip® Scanner 3000.
|
Description |
Gene expression data from Ramos cell line
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Data processing |
The data were normalized with R 2.15 and Bioconductor 2.10 software using RMA algorithm.
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|
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Submission date |
Aug 16, 2012 |
Last update date |
Jun 01, 2013 |
Contact name |
Victor Segura |
E-mail(s) |
vsegura@unav.es
|
Organization name |
FIMA
|
Department |
Unit of Proteomics, Genomics and Bioinformatics
|
Street address |
Avda. Pío XII, 55
|
City |
Pamplona |
State/province |
Navarra |
ZIP/Postal code |
31008 |
Country |
Spain |
|
|
Platform ID |
GPL6244 |
Series (1) |
GSE40168 |
Expression profile of MCF7, CCD18 and Ramos human cell lines |
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