|
| Status |
Public on Aug 10, 2012 |
| Title |
Cyanase 1U |
| Sample type |
SRA |
| |
|
| Source name |
intact frozen liver tissue
|
| Organism |
Mus musculus |
| Characteristics |
restriction enzyme: Cyanase 1U
strain: C57BL/6
tissue: Liver
age: 8 week
gender: male
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
The TACh method uses pulverized frozen tissue as starting material and employs one of the two robust endonucleases, Benzonase or Cyansase, which are fully active under a range of stringent conditions such as high levels of detergent and DTT. As a proof of principle we applied TACh to frozen mouse liver tissue.
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
Bed: Areas of enrichment were identified using a newly developed hotspot algorithm
Genome_build: mm9 (NCBI 37)
Supplementary_files_format_and_content: Bed
|
| |
|
| Submission date |
Aug 08, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Songjoon Baek |
| Organization name |
NCI / NIH
|
| Department |
CCR
|
| Lab |
LRBGE
|
| Street address |
41 Library Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL11002 |
| Series (1) |
| GSE39982 |
Rapid Genome-Scale Mapping of Chromatin Accessibility in Tissue |
|
| Relations |
| SRA |
SRX174760 |
| BioSample |
SAMN01109463 |
