|
| Status |
Public on Nov 05, 2012 |
| Title |
Patient 21 pre-treatment serum |
| Sample type |
protein |
| |
|
| Source name |
Serum
|
| Organism |
Homo sapiens |
| Characteristics |
cancer: hormone refractory prostate cancer
designation: Non-responder
treatment status: pre
protein: Human IgG
|
| Extracted molecule |
protein |
| Extraction protocol |
Serum was obtained from the supernatant of whole blood with the red blood cells spun down and was frozen in aliquots and stored at -80C. Protein is purified from baculovirus using either GST or His tags.
|
| Label |
Alexafluor 647
|
| Label protocol |
Commercially available anti-human IgG conjugated with Alexafluor647 was purchased from Invitrogen.
|
| |
|
| Hybridization protocol |
Serum was diluted 1:500 and used to hybridize Invitrogen protoarray v4.0. Bound antibodies were detected with anti-human IgG conjugated with Alexafluor 647.
|
| Scan protocol |
Arrays were scanned with an Axon scanner.
|
| Description |
Serum was diluted 1:500 and used to hybridize Invitrogen protoarray v4.0. Bound antibodies were detected with anti-human IgG conjugated with Alexafluor 647. Array was scanned with an Axon scanner.
|
| Data processing |
The raw data for all arrays were quantile-normalized, median-centered, and log2 transformed using R version 2.14.1
|
| |
|
| Submission date |
Jul 26, 2012 |
| Last update date |
Nov 05, 2012 |
| Contact name |
Serena S Kwek |
| E-mail(s) |
serena.kwek@ucsf.edu
|
| Phone |
4155149301
|
| Organization name |
UCSF
|
| Department |
Medicine
|
| Lab |
Hem/Onc
|
| Street address |
513 Parnassus Ave.
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL15855 |
| Series (1) |
| GSE39688 |
Immune response biomarker profiling of serum from prostate cancer patients treated with anti-CTLA-4 immunotherapy. |
|
