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Sample GSM973440 Query DataSets for GSM973440
Status Public on Jul 26, 2012
Title wt heart 1
Sample type RNA
 
Source name heart muscle, wild type
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: wild type
tissue: heart muscle
Extracted molecule total RNA
Extraction protocol RNA was extracted by using RNeasy Midi Kit (Qiagen) following manufacturers instructions for animal tissues.
Label biotin
Label protocol Biotinylated cDNA was prepared according to the Affymetrix Whole Transkript Sense Labelling protocol from100 ng total RNA according to the manufacturer
 
Hybridization protocol Following fragmentation, 15 ug of cDNA were hybridized for 16 hr at 45°C on GeneChip MoGene-1.0-st-v1 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GCS3000 gene chip scanner with GCOS 1.4.
Data processing The data were analysed using Bioconductor package 'xps' under R-2.10.0. Background correction, normalization and probe summarization was performed with RMA for each tissue separately using the core transcript annotation.
 
Submission date Jul 25, 2012
Last update date Jul 26, 2012
Contact name Stephan Direnberger
E-mail(s) direnberger@bio.lmu.de
Organization name Ludwig-Maximilians University
Street address Grosshaderner Str. 2
City Martinsried
ZIP/Postal code 82152
Country Germany
 
Platform ID GPL11533
Series (1)
GSE39632 Gene Expression profiling of transgenic mice expressing the genetically encoded calcium indicator TN-XXL in muscle and brain tissues

Data table header descriptions
ID_REF
VALUE RMA log2 transformed signal values.

Data table
ID_REF VALUE
10338001 9.687165694
10338003 8.176432487
10338004 7.766502406
10338017 11.27530975
10338025 6.532404242
10338026 11.5579375
10338029 7.627059596
10338035 6.547357282
10338036 7.590886411
10338037 1.689987911
10338041 9.122294811
10338042 8.424187289
10338044 10.54887006
10338047 3.264321737
10338056 1.511407314
10338059 11.08551229
10338060 1.643117341
10338064 1.977734186
10338065 3.214162116
10338066 1.714566447

Total number of rows: 35382

Table truncated, full table size 721 Kbytes.




Supplementary file Size Download File type/resource
GSM973440_E10R025c01.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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