|
| Status |
Public on Jul 25, 2013 |
| Title |
28260_KSO_M_KY |
| Sample type |
SRA |
| |
|
| Source name |
muscle
|
| Organism |
Bos taurus |
| Characteristics |
breed: Hanwoo/coreanae
gender type: castrated male
age: 30 months
feeding barn: Hanwoo Experimental Station in National Institute of Animal Science
tissue: muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using GeneAll Hybrid-RTM Kit (Cat# 305-101) from each tissues. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 5 ug of total RNA for the construction of sequencing libraries.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
mRNA-Sequencing
|
| Data processing |
Quality of RNA-seq reads from all of the adipose tissues were checked using FastQC.
Reads passed the quality control were mapped to Bovine Taurus genome (bosTau6) from UCSC using Tophat2 (v2.0.2).
The reads were counted using HTseq (v0.5.3p3).
Genome_build: bosTau6
Supplementary_files_format_and_content: tab-delimited text files include raw counts of genes (abundance measurements) for each sample.
|
| |
|
| Submission date |
Jul 24, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
HYEONGMIN KIM |
| E-mail(s) |
lazenca01@gmail.com
|
| Phone |
082-10-7147-6494
|
| Organization name |
Seoul National University
|
| Department |
College of Agriculture Sciences
|
| Lab |
biopop
|
| Street address |
Seoul National Univ., Daehak-dong, Gwanak-gu,
|
| City |
Seoul |
| ZIP/Postal code |
151-921 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL15749 |
| Series (1) |
| GSE39618 |
Comparative transcriptome analysis of various adipose depots in Hanwoo |
|
| Relations |
| SRA |
SRX170747 |
| BioSample |
SAMN01093734 |
