NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM953100 Query DataSets for GSM953100
Status Public on Jun 27, 2012
Title COPD [122541_mRNA]
Sample type RNA
 
Source name Lung tissue from smoker with COPD
Organism Homo sapiens
Characteristics diagnosis: COPD
fvc-group: 1
gold stage: 4
age: 55
gender (1=male, 2=female): 2
pkyrs: 37
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from frozen tissue samples using a freeze fracture protocol, followed by Trizol extraction and precipitation at -20ÂșC overnight to increase the yield of small RNAs. RNA integrity was determined by capillary electrophoresis on an Agilent 2100 Bioanalyzer. Only RNA with an integrity number $7 was used in the profiling studies.
Label Cy5
Label protocol Samples were prepared for mRNA microarray analysis using Agilent Quick Amp Labeling technologies (Santa Clara, California,USA).
 
Hybridization protocol Labeling was performed as described in Agilent protocol
Scan protocol Hybridized slides were washed and scanned with ScanArray Express (Perkin Elmer)
Description COPD
Data processing Gene expression data was normalized by quantile method, and transformed in log2-scale
 
Submission date Jun 27, 2012
Last update date Jun 27, 2012
Contact name Ji-Hoon Cho
E-mail(s) jcho@systemsbiology.org
Organization name Institute for Systems Biology
Street address 401 Terry Avenue North
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL4133
Series (1)
GSE38974 Gene expression networks in COPD: microRNA and mRNA regulation

Data table header descriptions
ID_REF
VALUE Log2-normalized (quantile normalization) signal intensity

Data table
ID_REF VALUE
1 14.961219
2 4.6690228
3 3.819543
4 4.5204689
5 3.7580989
6 4.7467248
7 4.068977
8 3.7697004
9 3.5009233
10 4.214436
11 3.502055
12 6.9551331
13 2.9979923
14 6.7578127
15 3.2022632
16 10.226879
17 3.9995197
18 5.4447594
19 11.598757
20 2.8733516

Total number of rows: 45220

Table truncated, full table size 690 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap