|
| Status |
Public on Jan 01, 2013 |
| Title |
P_1 with array type Medicago from Affymetrix |
| Sample type |
RNA |
| |
|
| Source name |
low input of phosphate 130µM,not inoculated, first replicate
|
| Organism |
Medicago truncatula |
| Characteristics |
cultivar: Jemalong
genotype: A17
age: 4 weeks
|
| Treatment protocol |
Name:TRAITEMENT 1 - environmental treatment - abiotic stress,low p, normal n, non inoculated:quantity 130uM . Factor value : 130 µM P, 6 mM NH4NO3, non inoculated Medicago truncatula seedlings were transferred on sand (that was washed and baked at 180°C for 14 h) in the presence of non inoculated leek roots. Plants were cultivated for 4 weeks and watered 3 times a week with a modified Long Ashton solution containing 130 µM phosphate and full N (6 mM NH4NO3).
|
| Growth protocol |
roots - on sand - in chamber: 22°C light (380 µE m-2 s-1) for 16 h, 19°C dark for 8h -
|
| Extracted molecule |
total RNA |
| Extraction protocol |
P_1:15ug.
|
| Label |
Biotin
|
| Label protocol |
labelling Biotin indirect, amplification=yes, ssDNA 5.5 ug.
|
| |
|
| Hybridization protocol |
labelled extract quantity: 15ug
|
| Scan protocol |
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
|
| Description |
Biological question (15 lines max): In natural ecosystems most vascular plants develop symbiosis with arbuscular mycorrhizal (AM) fungi which help them acquire nutrients such as phosphorus (P) and nitrogen (N). P has long been known to control AM symbiosis which takes place only when P is limiting. For N, however, its role in controlling mycorrhization is less clear. We have chosen the model plant Medicago truncatula to analyze the impact of P limitation and both P and N limitation on Medicago root transcriptome in response to the AM fungus Rhizophagus irregularis (formerly Glomus intraradices (BEG141)). These analyses may help us uncover signaling events involved in the interaction between these symbionts as well as genes encoding transporters potentially important for nutrient exchanges in these conditions. -
|
| Data processing |
The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
|
| |
|
| Submission date |
Jun 21, 2012 |
| Last update date |
Jan 01, 2013 |
| Contact name |
stephanie huguet |
| E-mail(s) |
huguet@evry.inra.fr
|
| Organization name |
inra
|
| Street address |
2 rue gaston cremieux
|
| City |
evry |
| ZIP/Postal code |
91000 |
| Country |
France |
| |
|
| Platform ID |
GPL4652 |
| Series (1) |
| GSE38847 |
affy_med_2011_09-Analysis of Medicago truncatula root transcriptome in response to mycorrhization by Glomus intraradices under phosphate and nitrogen limitation |
|
