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Sample GSM945854 Query DataSets for GSM945854
Status Public on Jun 08, 2012
Title USC_ChipSeq_MCF-7_H3K27ac_UCDavis
Sample type SRA
 
Source name MCF-7
Organism Homo sapiens
Characteristics lab: USC
lab description: Farnham - University of Southern California
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell: MCF-7
cell organism: human
cell description: mammary gland, adenocarcinoma. (PMID: 4357757), newly promoted to tier 2: not in 2011 analysis
cell karyotype: cancer
cell lineage: ectoderm
cell sex: F
treatment: None
treatment description: No special treatment or protocol applies
antibody: H3K27ac
antibody antibodydescription: rabbit polyclonal. Antibody Target: H3K27ac
antibody targetdescription: Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname: Abcam
antibody vendorid: ab4729
control: UCDavis
control description: Input library was prepared at UC Davis.
control: UCDavis
control description: Input library was prepared at UC Davis.
controlid: wgEncodeEH000762
replicate: 1
Biomaterial provider ATCC
Treatment protocol None
Growth protocol MCF-7_Farnham_protocol.pdf
Extracted molecule genomic DNA
Extraction protocol http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhHistone
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer
 
Data processing http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhHistone
 
Submission date Jun 08, 2012
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL9052
Series (2)
GSE31755 Histone Modifications by ChIP-seq from ENCODE/Stanford/Yale/Davis/Harvard
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
Relations
SRA SRX152075
BioSample SAMN01041455
Named Annotation GSM945854_hg19_wgEncodeSydhHistoneMcf7H3k27acUcdSig.bigWig

Supplementary file Size Download File type/resource
GSM945854_hg19_wgEncodeSydhHistoneMcf7H3k27acUcdPk.narrowPeak.gz 465.6 Kb (ftp)(http) NARROWPEAK
GSM945854_hg19_wgEncodeSydhHistoneMcf7H3k27acUcdSig.bigWig 257.0 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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