|
Status |
Public on Jun 08, 2012 |
Title |
USC_ChipSeq_MCF-7_H3K27ac_UCDavis |
Sample type |
SRA |
|
|
Source name |
MCF-7
|
Organism |
Homo sapiens |
Characteristics |
lab: USC lab description: Farnham - University of Southern California datatype: ChipSeq datatype description: Chromatin IP Sequencing cell: MCF-7 cell organism: human cell description: mammary gland, adenocarcinoma. (PMID: 4357757), newly promoted to tier 2: not in 2011 analysis cell karyotype: cancer cell lineage: ectoderm cell sex: F treatment: None treatment description: No special treatment or protocol applies antibody: H3K27ac antibody antibodydescription: rabbit polyclonal. Antibody Target: H3K27ac antibody targetdescription: Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases. antibody vendorname: Abcam antibody vendorid: ab4729 control: UCDavis control description: Input library was prepared at UC Davis. control: UCDavis control description: Input library was prepared at UC Davis. controlid: wgEncodeEH000762 replicate: 1
|
Biomaterial provider |
ATCC
|
Treatment protocol |
None
|
Growth protocol |
MCF-7_Farnham_protocol.pdf
|
Extracted molecule |
genomic DNA |
Extraction protocol |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhHistone
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina Genome Analyzer |
|
|
Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhHistone
|
|
|
Submission date |
Jun 08, 2012 |
Last update date |
May 15, 2019 |
Contact name |
ENCODE DCC |
E-mail(s) |
encode-help@lists.stanford.edu
|
Organization name |
ENCODE DCC
|
Street address |
300 Pasteur Dr
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305-5120 |
Country |
USA |
|
|
Platform ID |
GPL9052 |
Series (2) |
GSE31755 |
Histone Modifications by ChIP-seq from ENCODE/Stanford/Yale/Davis/Harvard |
GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
|
Relations |
SRA |
SRX152075 |
BioSample |
SAMN01041455 |
Named Annotation |
GSM945854_hg19_wgEncodeSydhHistoneMcf7H3k27acUcdSig.bigWig |