|
| Status |
Public on Jun 08, 2012 |
| Title |
USC_ChipSeq_HCT-116_H3K27ac_UCDavis |
| Sample type |
SRA |
| |
|
| Source name |
HCT-116
|
| Organism |
Homo sapiens |
| Characteristics |
lab: USC
lab description: Farnham - University of Southern California
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell: HCT-116
cell organism: human
cell description: colorectal carcinoma (PMID: 7214343)
cell karyotype: cancer
cell lineage: endoderm
cell sex: M
treatment: None
treatment description: No special treatment or protocol applies
antibody: H3K27ac
antibody antibodydescription: rabbit polyclonal. Antibody Target: H3K27ac
antibody targetdescription: Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname: Abcam
antibody vendorid: ab4729
control: UCDavis
control description: Input library was prepared at UC Davis.
control: UCDavis
control description: Input library was prepared at UC Davis.
controlid: wgEncodeEH000627
replicate: 1
|
| Biomaterial provider |
ATCC
|
| Treatment protocol |
None
|
| Growth protocol |
HCT-116_protocol.pdf
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhHistone
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhHistone
|
| |
|
| Submission date |
Jun 08, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL9052 |
| Series (1) |
| GSE31755 |
Histone Modifications by ChIP-seq from ENCODE/Stanford/Yale/Davis/Harvard |
|
| Relations |
| SRA |
SRX152074 |
| BioSample |
SAMN01041454 |
| Named Annotation |
GSM945853_hg19_wgEncodeSydhHistoneHct116H3k27acUcdSig.bigWig |
