 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 22, 2012 |
| Title |
Harvard_ChipSeq_MCF10A-Er-Src_4OHTAM_1uM_12hr_STAT3_Harvard_Control |
| Sample type |
SRA |
| |
|
| Source name |
MCF10A-Er-Src
|
| Organism |
Homo sapiens |
| Characteristics |
lab: Harvard
lab description: Struhl - Harvard University
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell: MCF10A-Er-Src
cell organism: human
cell description: mammary gland, non-tumorigenic epithelial, inducible cell line, derived from the MCF-10A parental cells and contain ER-Src, a derivative of the Src kinase oncoprotein (v-Src) that is fused to the ligand-binding domain of the estrogen receptor (ER)
cell karyotype: normal
cell lineage: ectoderm
cell sex: F
treatment: 4OHTAM_1uM_12hr
treatment description: 12 hours with 1 uM 4-hydroxytamoxifen dissolved in ethanol (Struhl)
antibody: STAT3
antibody antibodydescription: Rabbit polyclonal; epitope mapping at the C-terminus of Stat3 of mouse origin. Antibody Target: STAT3
antibody targetdescription: Member of STAT family. After phosphorylation in response to cytokines and growth factors (including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2), forms homo- or heterodimers that translocate to nucleus and activate transcription. Activates expression of a variety of genes with roles in processes such as cell growth and apoptosis.
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-482
control: Harvard_Control
control description: input library was prepared at Harvard.
control: Harvard_Control
control description: input library was prepared at Harvard.
controlid: wgEncodeEH002871
replicate: 1
|
| Biomaterial provider |
Struhl laboratory
|
| Treatment protocol |
4OHTAM_1uM_12hr
|
| Growth protocol |
MCF-10A_Struhl_protocol.pdf
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhTfbs
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeSydhTfbs
|
| |
|
| Submission date |
May 22, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL9052 |
| Series (1) |
| GSE31477 |
ENCODE Transcription Factor Binding Sites by ChIP-seq from Stanford/Yale/USC/Harvard |
|
| Relations |
| SRA |
SRX150479 |
| BioSample |
SAMN01000939 |
| Named Annotation |
GSM935399_hg19_wgEncodeSydhTfbsMcf10aesStat3Tam112hHvdSig.bigWig |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM935399_hg19_wgEncodeSydhTfbsMcf10aesStat3Tam112hHvdPk.narrowPeak.gz |
1.6 Mb |
(ftp)(http) |
NARROWPEAK |
| GSM935399_hg19_wgEncodeSydhTfbsMcf10aesStat3Tam112hHvdSig.bigWig |
255.0 Mb |
(ftp)(http) |
BIGWIG |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
