|
| Status |
Public on May 23, 2012 |
| Title |
Abdomen_Control Population 2_Day 1_Rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Abdomen_control_1 day of age
|
| Organism |
Drosophila melanogaster |
| Characteristics |
gender: female
group: control populations
tissue: Abdomen
population: 2C
age (day): 1
biological replicate: 2
|
| Treatment protocol |
We established three independent pairs of selection-control populations originally derived from an ancestral population of wild-caught female flies collected in New Jersey in 1998. Flies in the control populations were raised in single-sex vials and allowed to mate at 14 days of age.
Flies in the selection populations were also raised in single sex vials and allowed to mate initially at 28 days of age and later at 40 days of age as flies evolved longer reproductive lifespans.
After 35 generations of imposing selection for longer reproductive lifespan, unmated female offspring of control and selection populations were allowed to age to 5 different age classes and were collected, frozen and dissected into two separate tissues for gene expression analysis.
|
| Growth protocol |
Drosophila melanogaster flies were maintained in an incubator at 25 degrees celsius on a 12L:12D light cycle and transferred to fresh cornmeal media every week.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the PicoPure RNA isolation kit (Arcturus), and DNA was removed by on-column DNase digestion with the RNase-Free DNase set (Qiagen). RNA was amplified using Ambion's Message AMpII RNA amplification Kit (Ambion). RNA was then reversed trasncribed to cDNA using SuperScript III (Invitrogen).
|
| Label |
Cy 3
|
| Label protocol |
Labeling was performed by Florida State University NimbleGen Systems Inc. approved facility following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by Florida State University NimbleGen Systems Inc. approved facility following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by Florida State University NimbleGen Systems Inc. approved facility following their standard operating protocol. See www.nimblegen.com.
|
| Description |
SAMPLE 32
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
May 22, 2012 |
| Last update date |
May 23, 2012 |
| Contact name |
Silvia Remolina |
| Organization name |
University of Illinois
|
| Street address |
505 S Goodwin Ave
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
|
| Platform ID |
GPL15486 |
| Series (1) |
| GSE38106 |
Expression analysis of D. melanogaster selected for contrasting life histories |
|
