|
| Status |
Public on May 03, 2012 |
| Title |
cord blood CD34+ from ARV-exposed newborns_A20 |
| Sample type |
RNA |
| |
|
| Source name |
cord blood cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment protocol: ARV-exposed newborns
tissue: cord blood
cell type: CD34+ hematopoietic progenitors
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cord blood was collected during the third stage of labor with the placenta in utero.Mononuclear cells were separated out, and the CD34+ cell fraction was magnetically sorted.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA ( GeneChip 3'IVT Express Technical Manual, 2008, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HG-U133 Plus 2.0 GeneChip arrays . GeneChips were washed and stained in the Affymetrix Fluidics Station 400
|
| Scan protocol |
GeneChips were scanned using the Affymetrix scanner 3000
|
| Description |
Gene expression data from cord blood-derived mononuclear cells from HIV-1 uninfected newborn exposed in utero to a zidovudine-based ARV
|
| Data processing |
All data analyses were performed in R using Bioconductor libraries and R statistical packages.
Probe signals were converted to expression values using the robust multi-array average procedure (RMA)
|
| |
|
| Submission date |
May 03, 2012 |
| Last update date |
May 03, 2012 |
| Contact name |
Fabienne Cocchiarella |
| E-mail(s) |
fabienne.cocchiarella@unimore.it
|
| Phone |
+39 0592058062
|
| Fax |
+39 0592058116
|
| Organization name |
università di Modena e Reggio Emilia
|
| Department |
Scienze Biomediche
|
| Street address |
Via G. Gottardi
|
| City |
Modena |
| ZIP/Postal code |
41125 |
| Country |
Italy |
| |
|
| Platform ID |
GPL14837 |
| Series (1) |
| GSE37748 |
Genotoxic alterations of cord blood cells in newborns exposed in utero to a zidovudine-based antiretroviral combination |
|
