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|
| Status |
Public on Dec 30, 2006 |
| Title |
NOD Dbl single positive rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse thymocyte
|
| Organism |
Mus musculus |
| Characteristics |
NOD diabetic, 6-8 week old, double transgenic TCR:insHEL, CD4+ thymocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from sorted thymus using trizol and further purified using RNeasy spin columns.
|
| Label |
biotin
|
| Label protocol |
Affymetrix GeneChip Eukaryotic Small Sample Target Labelling Assay Version II protocol was used on total RNA from 100000-1000000 cells
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
NOD negative selection CD4+ thymocytes
|
| Data processing |
Affymetrix Microarray Suite version 5.0
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| |
|
| Submission date |
Jan 09, 2006 |
| Last update date |
Jul 21, 2006 |
| Contact name |
Kristine Hardy |
| E-mail(s) |
kristine.hardy@anu.edu.au
|
| Organization name |
University of Canberra
|
| Lab |
Cytokine Gene Expression
|
| Street address |
University of Canberra
|
| City |
Bruce |
| State/province |
ACT |
| ZIP/Postal code |
0200 |
| Country |
Australia |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE3997 |
Impairment of organ-specific T cell negative selection by diabetes susceptibility genes: analysis by mRNA profiling |
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| Data table header descriptions |
| ID_REF |
|
| VALUE |
MAS5 calculated Signal intensity |
| ABS_CALL |
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC) |
| DETECTION P-VALUE |
'detection p-value', p-value that indicates the significance level of the detection call |
| Data table |
| ID_REF |
VALUE |
ABS_CALL |
DETECTION P-VALUE |
| AFFX-BioB-5_at |
71.4 |
P |
0.002867 |
| AFFX-BioB-M_at |
120.3 |
P |
0.000044 |
| AFFX-BioB-3_at |
82.5 |
P |
0.000095 |
| AFFX-BioC-5_at |
219.8 |
P |
0.000169 |
| AFFX-BioC-3_at |
170.4 |
P |
0.000044 |
| AFFX-BioDn-5_at |
215.2 |
P |
0.000044 |
| AFFX-BioDn-3_at |
980 |
P |
0.00007 |
| AFFX-CreX-5_at |
2157.1 |
P |
0.000044 |
| AFFX-CreX-3_at |
3836.9 |
P |
0.000044 |
| AFFX-DapX-5_at |
5.1 |
A |
0.195266 |
| AFFX-DapX-M_at |
11 |
A |
0.102165 |
| AFFX-DapX-3_at |
0.8 |
A |
0.945787 |
| AFFX-LysX-5_at |
3 |
A |
0.354453 |
| AFFX-LysX-M_at |
6.5 |
A |
0.559354 |
| AFFX-LysX-3_at |
6.6 |
A |
0.185131 |
| AFFX-PheX-5_at |
1.2 |
A |
0.891021 |
| AFFX-PheX-M_at |
0.9 |
A |
0.794268 |
| AFFX-PheX-3_at |
23.7 |
A |
0.116113 |
| AFFX-ThrX-5_at |
1.5 |
A |
0.824672 |
| AFFX-ThrX-M_at |
2.1 |
A |
0.659339 |
Total number of rows: 22690
Table truncated, full table size 606 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM91302.CEL.gz |
3.4 Mb |
(ftp)(http) |
CEL |
| GSM91302.EXP.gz |
492 b |
(ftp)(http) |
EXP |
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