|
| Status |
Public on Jan 22, 2013 |
| Title |
H9 PluriSIn #1 (1) |
| Sample type |
RNA |
| |
|
| Source name |
H9, PluriSIn #1
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: undifferentiated human embryonic stem cells
genotype/variation: 46(X,X)
age: passage 52
cell line: H9
treatment: PluriSIn #1
|
| Treatment protocol |
Undifferentiated pluripotent stem cells were grown on matrigel with mTeSR1 and exposed to PluriSIn #1 at 20uM with 0.02% DMSO for 12 h.
|
| Growth protocol |
Cells were grown on matrigel-coated plates using mTeSR1 defined medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using 5 Prime RNA isolation kit.
|
| Label |
biotin
|
| Label protocol |
200ng total RNA, Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Affymetrix protocol, 11ug cRNA.
|
| Scan protocol |
GeneChip scanner 3000 7G, GeneChip Operating Software v1.4.
|
| Description |
Gene expression data from undifferentiated pluripotent stem cells grown on matrigel with mTeSR1 and exposed to PluriSIn #1 at 20uM with 0.02% DMSO for 12 h.
|
| Data processing |
Affymetrix Expression Console MAS5.
|
| |
|
| Submission date |
Apr 04, 2012 |
| Last update date |
May 22, 2014 |
| Contact name |
Uri Ben-David |
| E-mail(s) |
uri.bendavid@gmail.com
|
| Phone |
97226585185
|
| Organization name |
Hebrew University
|
| Department |
Genetics
|
| Lab |
Nissim Benvenisty
|
| Street address |
Givat-Ram
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE37040 |
Expression data from human pluripotent stem cells and differentiated cells treated with pluripotent-specific inhibitors |
|
