|
| Status |
Public on Jul 13, 2012 |
| Title |
H1 replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
ES, WA01
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: F,embryonic
cell line: WA01
|
| Treatment protocol |
Fibroblasts were detached from tissue flasks using trypsin and iPSCs and HUES6 were detached manually. After that, cells were collected with media, centrifuged and ressuspended in Trizol.
|
| Growth protocol |
iPSCs and HUES6 were grown in mTeSR media at 37oC in a humidified atmosphere at 5% CO2. Fibroblasts were maintained in DMEM containing 15% Fetal Bovine Serum also at 37oC in a humidified atmosphere at 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol according manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
Gene expression data from Human Embryonic Stem cell (replicate 1)
|
| Data processing |
HuGene-1.0-st were analyzed by using affymetrix power tools (apt-probeset-summarize -a rma-sketch -a plier-mm-sketch -d ../../Human/CD_HG-U133_Pl us_2_Libfiles/HG-U133_Plus_2.cdf -o gene-level-output CM0811101A.CEL CM0811101B.CEL CM0811101C.CEL CM0811102A.CEL CM08111 02B.CEL CM0811102C.CEL CM081110CA.CEL CM081110CB.CEL CM081110CC.CEL CM081110HA.CEL CM081110HB.CEL CM081110HC.CEL CM081110 RA.CEL CM081110RB.CEL CM081110RC.CEL )
|
| |
|
| Submission date |
Mar 20, 2012 |
| Last update date |
Jul 13, 2012 |
| Contact name |
Jason Nathanson |
| E-mail(s) |
nathanson@ucsd.edu
|
| Organization name |
University of Californa San Diego
|
| Department |
Cellular and Molecular Medicine
|
| Lab |
Gene Yeo
|
| Street address |
2880 Torrey Pines Rd, Rm 3807
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE36648 |
Expression data in induced pluripotent stem cells (iPSCs) derived from a DNA repair deficient fibroblast |
|
