|
| Status |
Public on Mar 17, 2012 |
| Title |
LNCaP95_ControlsiRNA_CSS |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
LNCaP95 cells transfected with control siRNA and cultured in androgen depleted conditions
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCaP95
genotype/variation: transfected with control siRNA
culture conditions/treatment: cultured in androgen depleted conditions
tissue: Prostate
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
2 µg of total RNA were processed accoridng to instructions in the Agilent Quick AMP Labeling Kit
|
| |
|
| Channel 2 |
| Source name |
Total RNA from LNCaP cells grown in normal culture conditions
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCaP
reference: Total RNA from LNCaP cells grown in normal culture conditions
tissue: Prostate
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
2 µg of total RNA were processed accoridng to instructions in the Agilent Quick AMP Labeling Kit
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequentially using washing buffers recommened by Agilent.
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner
|
| Description |
Treatment with siRNA for 24 hours followed by 48 hours in specified culture conditions
|
| Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization
|
| |
|
| Submission date |
Mar 15, 2012 |
| Last update date |
Mar 18, 2012 |
| Contact name |
Jun Luo |
| E-mail(s) |
jluo1@jhmi.edu
|
| Organization name |
Johns Hopkins University
|
| Street address |
600 N Wolfe st
|
| City |
baltimore |
| State/province |
MD |
| ZIP/Postal code |
21287 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE36549 |
Expression correlates of the full-length androgen receptor and its splicing variants |
|
