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Sample GSM886 Query DataSets for GSM886
Status Public on Feb 12, 2002
Title PHO81c vs. wild type, exp.2
Sample type RNA
 
Channel 1
Source name Cy3-labeled cDNA from NBW7 mRNA in YPAD
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name Cy5-labeled cDNA from NOF1(PHO81c) mRNA in YPAD
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description NBW7 (MATa ade2 his3 leu2 trp1 ura3 pho3-1), a wildtype strain for the PHO-regulation study. NBD82-1 (MATa ade2 his3 leu2 trp1 ura3 pho3-1 PHO81c-1). PHO81c-1 is a dominant mutation allele on the PHO81 locus. [Ref., Ogawa et al., (1995) Mol Cell Biol 15:997-1004] RNA samples were prepared from their logarithmic phase cultures (OD600 = 0.5). This data was converted from RANA database.
ScanAlyze version 2.41 scanning software was used.
Keywords = Phosphate
Keywords = Nutrient limitation
Lot batch = pho812
 
Submission date Feb 08, 2002
Last update date Nov 21, 2005
Organization Stanford Microarray Database (SMD)
E-mail(s) array@genome.stanford.edu
Phone 650-498-6012
URL http://genome-www5.stanford.edu/
Department Stanford University, School of Medicine
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL66
Series (1)
GSE20 Manipulation in phospate levels

Data table header descriptions
ID_REF reference identifier
CH1B_MEAN mean of channel 1 background pixels
CH1B_MEDIAN median of channel 1 background pixels
CH1D_MEAN difference between CH1I_MEAN and CH1B_MEAN
CH2I_MEAN mean of channel 2 signal pixels
CH2D_MEAN difference of CH2I_MEAN and CH2B_MEAN
CH2B_MEAN mean of channel 2 background pixels
CH2B_MEDIAN median of channel 2 background pixels
CH2BN_MEDIAN normalized CH2B_MEDIAN
CH2DN_MEAN normalized CH2D_MEAN
CH2IN_MEAN normalized CH2I_MEAN
CORR simple correlation coefficient of channel 1 and channel 2 pixels
FLAG user defined flag (default=0)
VALUE same as UNF_VALUE but with flagged values removed
PIX_RAT2_MEDIAN
PERGTBCH1I_1SD standard deviation of fraction of pixels greater than CH1B_MEAN
PERGTBCH2I_1SD standard deviation of fraction of pixels greater than CH2B_MEAN
RAT1_MEAN ratio of CH1D_MEAN to CH2D_MEAN
RAT1N_MEAN ratio of CH1DN_MEAN to CH2DN_MEAN
RAT2_MEAN arithmetic mean of ratios of CH2D_MEAN to CH1D_MEAN
RAT2N_MEAN arithmetic mean of ratios of CH2DN_MEAN to CH1DN_MEAN
REGR slope of the simple regression line of channel 2 to channel 1 pixels
TOT_BPIX total number of background pixels
TOT_SPIX total number of signal (spot) pixels
TOP vertical, short-axis spot ellipse minimum pixel coordinate
BOT vertical, short-axis spot ellipse maximum pixel coordinate
LEFT horizontal, long-axis spot ellipse minimum pixel coordinate
RIGHT horizontal, long-axis spot ellipse maximum pixel coordinate
UNF_VALUE aka LOG_RAT2N_MEAN, or log2 of ratio of CH2DN_MEAN and CH1DN_MEAN

Data table
ID_REF CH1B_MEAN CH1B_MEDIAN CH1D_MEAN CH2I_MEAN CH2D_MEAN CH2B_MEAN CH2B_MEDIAN CH2BN_MEDIAN CH2DN_MEAN CH2IN_MEAN CORR FLAG VALUE PIX_RAT2_MEDIAN PERGTBCH1I_1SD PERGTBCH2I_1SD RAT1_MEAN RAT1N_MEAN RAT2_MEAN RAT2N_MEAN REGR TOT_BPIX TOT_SPIX TOP BOT LEFT RIGHT UNF_VALUE
1 2152 1816 3404 4720 3864 1091 856 1297 5855 7152 .975 0 .782 1.179 25 43 .881 .581 1.135 1.72 1.02 1487 32 21 27 122 128 .782
2 2222 1840 5819 6177 5313 1137 864 1309 8050 9359 .979 0 .468 .898 65 68 1.095 .723 .913 1.383 .892 1419 32 21 27 132 138 .468
3 2238 1840 6238 7570 6698 1148 872 1321 10149 11470 0 0 .702 1.11 53 62 .931 .615 1.074 1.627 0 1399 32 21 27 142 148 .702
4 2056 1776 7087 7403 6563 1031 840 1273 9944 11217 0 0 .489 1.052 53 56 1.08 .713 .926 1.403 0 1399 32 21 27 152 158 .489
5 1801 1720 6860 8281 7481 855 800 1212 11335 12547 0 0 .725 1.055 46 50 .917 .605 1.091 1.652 0 1399 32 21 27 162 168 .725
6 1718 1688 6629 8009 7217 809 792 1200 10935 12135 0 0 .722 1.055 46 53 .919 .606 1.089 1.65 0 1399 32 21 27 172 178 .722
7 1691 1680 5612 6427 5635 800 792 1200 8538 9738 .965 0 .605 1.1 46 53 .996 .657 1.004 1.521 1.016 1399 32 21 27 182 188 .605
8 1700 1672 5602 6575 5791 807 784 1188 8774 9962 .993 0 .647 1.067 46 46 .967 .638 1.034 1.566 1.013 1389 32 21 27 192 198 .647
9 1699 1664 6566 7718 6950 805 768 1164 10530 11694 0 0 .681 1.04 43 53 .945 .624 1.058 1.604 0 1389 32 21 27 202 208 .681
10 1687 1656 46 827 67 796 760 1152 101 1253 .828 0 1.135 1.4 3 9 .687 .455 1.457 2.196 1.021 1334 32 23 29 210 216 1.135
11 1685 1648 12 742 788 752 1139 1124 .261 0 1.1 0 0 .19 1352 32 23 29 220 226
12 1689 1656 761 9 786 752 1139 14 1153 .085 0 1.2 0 0 .08 1364 32 23 29 230 236
13 1662 1656 537 1077 325 764 752 1139 493 1632 .936 0 -.123 .611 21 21 1.652 1.089 .605 .918 .658 1359 32 23 29 243 249 -.123
14 1666 1656 1452 1221 461 768 760 1152 698 1850 .961 0 -1.057 .39 28 28 3.15 2.08 .317 .481 .357 1349 32 23 29 253 259 -1.057
15 1673 1664 252 962 202 775 760 1152 306 1458 .844 0 .28 .624 12 18 1.248 .824 .802 1.214 .991 1321 32 23 29 262 268 .28
16 1679 1672 1313 2030 1262 782 768 1164 1912 3076 .964 0 .542 .79 34 34 1.04 .687 .961 1.456 .923 1293 32 24 30 270 276 .542
17 1686 1672 748 784 768 1164 1133 -.199 0 .5 0 0 -.111 1300 32 24 30 280 286
18 1694 1680 46 759 788 768 1164 1150 .115 0 1.267 0 0 .066 1318 32 24 30 290 296
19 1696 1680 710 787 768 1164 1076 .045 0 .828 0 0 .03 1321 32 24 30 300 306
20 1691 1672 4 790 30 781 760 1152 45 1197 .358 0 3.492 2.125 0 3 .133 .089 7.5 11.25 .342 1321 32 24 30 310 316 3.492

Total number of rows: 6528

Table truncated, full table size 817 Kbytes.




Supplementary data files not provided
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