|
| Status |
Public on Feb 22, 2012 |
| Title |
placental tissue replicate 4 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
methylated CpG-island recovery assay (MIRA) enriched, placental tissue, 4 of 4 mother-baby pairs
|
| Organism |
Homo sapiens |
| Characteristics |
sample id: NMB8
tissue: placental tissue
sample type: methylation-enriched DNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated from fresh frozen tissues using standard phenol/chloroform method.
|
| Label |
cy5
|
| Label protocol |
The products of whole genome amplification from the total input DNA without MBD enrichment and from methylation-enriched DNA were purified by using DNA purification kit, respectively. The purified DNA fragments were then labeled with cy3-dCTP or cy5-dCTP with Klenow enzyme (Takara) according to the instruction of the manufacturer.
|
| |
|
| Channel 2 |
| Source name |
input, placental tissue, 4 of 4 mother-baby pairs
|
| Organism |
Homo sapiens |
| Characteristics |
sample id: NMB8
tissue: placental tissue
sample type: total input DNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated from fresh frozen tissues using standard phenol/chloroform method.
|
| Label |
cy3
|
| Label protocol |
The products of whole genome amplification from the total input DNA without MBD enrichment and from methylation-enriched DNA were purified by using DNA purification kit, respectively. The purified DNA fragments were then labeled with cy3-dCTP or cy5-dCTP with Klenow enzyme (Takara) according to the instruction of the manufacturer.
|
| |
|
| |
| Hybridization protocol |
According to the instructions of Agilent Oligo aCGH Hybridization Kit
|
| Scan protocol |
Scanned on an Agilent G2505C scanner.
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
| Description |
Sample name: NMB8-pla_cy5/cy3
Biological replicate 4 of 4 placental tissue
US10283824_252984210018_S01_CGH_107_Sep09_1_4.txt
|
| Data processing |
Agilent GeneSpring GX 11.5 was used for background subtraction and LOWESS normalization.Following global mean normalization, probes with an intensity <400 were discarded for further analysis.
|
| |
|
| Submission date |
Feb 22, 2012 |
| Last update date |
Feb 22, 2012 |
| Contact name |
wang liang |
| E-mail(s) |
wangliangkexue@163.com
|
| Organization name |
BioChainBJ
|
| Street address |
7A North Yongchang Road BDA
|
| City |
Beijing |
| ZIP/Postal code |
100176 |
| Country |
China |
| |
|
| Platform ID |
GPL14835 |
| Series (1) |
| GSE35997 |
DNA hypermethylation in placental tissues: placental tissues vs. maternal peripheral bloods |
|
