|
| Status |
Public on Feb 23, 2012 |
| Title |
Hep G2 vs. Hep G2-HGF |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Hep G2 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Hep G2 (HB-8065)
|
| Treatment protocol |
The experiment group is treated with HGF (10ng/ml) for 8 hours
|
| Growth protocol |
The cells are grown in serum-free DMEM for 24 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
0.2 μg of total RNA was amplified by a Low Input Quick-Amp Labeling kit (Agilent Technologies, USA) and labeled with Cy3 (CyDye, Agilent Technologies, USA) during the in vitro transcription process.
|
| |
|
| Channel 2 |
| Source name |
Hep G2 cells treated with HGF
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Hep G2 (HB-8065)
|
| Treatment protocol |
The experiment group is treated with HGF (10ng/ml) for 8 hours
|
| Growth protocol |
The cells are grown in serum-free DMEM for 24 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
0.2 μg of total RNA was amplified by a Low Input Quick-Amp Labeling kit (Agilent Technologies, USA) and labeled with Cy3 (CyDye, Agilent Technologies, USA) during the in vitro transcription process.
|
| |
|
| |
| Hybridization protocol |
0.6 μg of Cy3-labled cRNA was fragmented to an average size of about 50-100 nucleotides by incubation with fragmentation buffer at 60°C for 30 minutes. Correspondingly fragmented labeled cRNA is then pooled and hybridized to the microarray membrane at 65°C for 17 h.
|
| Scan protocol |
After washing and drying by nitrogen gun blowing, microarrays are scanned with an Agilent microarray scanner (Agilent Technologies, USA) at 535 nm for Cy3.
|
| Description |
Comparison of the transcriptional profile of HepG2 cells treated with HGF and control cells
|
| Data processing |
Scanned images are analyzed by Feature extraction10.5.1.1 software (Agilent Technologies, USA), an image analysis and normalization software is used to quantify signal and background intensity for each feature.
|
| |
|
| Submission date |
Feb 21, 2012 |
| Last update date |
Feb 23, 2012 |
| Contact name |
Yung-Ming Jeng |
| E-mail(s) |
mrna0912@yahoo.com.tw
|
| Phone |
886 2 23934172
|
| Fax |
886 2 23934172
|
| Organization name |
National Taiwan University Hospital
|
| Department |
Department of Pathology
|
| Street address |
7 Chung-Shan South Road
|
| City |
Taipei |
| ZIP/Postal code |
100 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE35994 |
HepG2 Cell: Control vs. HGF treated |
|
