|
| Status |
Public on Jan 31, 2025 |
| Title |
Electronic Cigarette Processing Group |
| Sample type |
SRA |
| |
|
| Source name |
Aorta
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Aorta
|
| Treatment protocol |
All animal studies were approved by the Capital Medical University (ethical review number: AEEI-2021-024). C57BL/6J male mice aged 8 weeks inhaled filtered air (as control) or e-cig (RELX, containing glycerin, propylene glycol, spices, and 3% nicotine) 1 h per day in the exposure chamber for consecutive 90 days.
|
| Growth protocol |
All mice were maintained under standard conditions (20‐24°C, 40%‐60% humidity, 12/12‐hour light/dark cycle).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The whole aorta samples collected from mice after exposure.
scRNA-seq libraries were constructed using the 10x Genomics Single Cell Gene Expression Solution v3.1. All the procedures were performed according to the standard manufacturer’s protocol (CG000315 Rev E). Briefly, beads with unique molecular identifier (UMI) and cell barcodes were loaded close to saturation, so that each cell was paired with a bead in a Gel Beads-in-emulsion (GEM). After exposure to cell lysis buffer, polyadenylated RNA molecules hybridized to the beads. Beads were retrieved into a single tube for reverse transcription. On cDNA synthesis, each cDNA molecule was tagged on the 3’end with UMI and cell label, indicating its cell of origin. Then cDNA along with cell barcodes were PCR-amplified.The constructed libraries were quantified using a High Sensitivity DNA Chip (Agilent) on a Bioanalyzer 4150 and the Qubit High Sensitivity DNA Assay (Thermo Fisher Scientific). Then the libraries were sequenced on NovaSeq 6000 (Illumina) with a sequencing depth of at least 20,000 reads per cell with a pair-end 150 bp (PE150) reading strategy.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Library name: E-cigs
10x Genomics
|
| Data processing |
The demultiplexing, barcoded processing, gene counting and aggregation w ere madeusing the Cell Ranger softw are v2.1.1
Assembly: Mouse mm10
Supplementary files format and content: Tab-separated values files and matrix files
|
| |
|
| Submission date |
Jan 24, 2025 |
| Last update date |
Jan 31, 2025 |
| Contact name |
唐 伟评 |
| Organization name |
Beijing Institute of Heart Lung and Blood Vessel Diseases
|
| Street address |
2 Anzhen Rd, Chaoyang District,
|
| City |
Beijing |
| ZIP/Postal code |
100029 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE288003 |
Single-cell transcriptomics revealed e-cigarettes-induced vascular remodeling by enhancing Tcf21 expression |
|
