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Status |
Public on Aug 02, 2012 |
Title |
SIN3A_siRNA_NS_rep3 |
Sample type |
RNA |
|
|
Source name |
MCF7 cells, SIN3A silenced, NS
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF7 breast cancer treatment: SIN3A silenced
|
Biomaterial provider |
Laura Icardi, Cytokine Receptor Lab, Prof. Jan Tavernier
|
Treatment protocol |
Transfected with 50nM SIN3A siRNA. 72h later, cells were cutured 4h without FCS
|
Growth protocol |
DMEM+10%FCS
|
Extracted molecule |
total RNA |
Extraction protocol |
Cell lysates were homogenized with the QiaShredder Column (Qiagen), and total cellular RNA was extracted with the Rneasy Mini kit (Qiagen) following manufacturer’s instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 nanogram total RNA.
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|
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Hybridization protocol |
Following fragmentation, 15 microgram of cRNA were hybridized for 17 hr at 45°C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
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Data processing |
Data were normalized using RMA as implemented in the Affy package (version 1.28.0) of Bioconductor. Expression values are represented on a log2-scale.
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Submission date |
Feb 10, 2012 |
Last update date |
Aug 02, 2012 |
Contact name |
Rekin's Janky |
E-mail(s) |
Nucleomics.Bioinformatics@vib.be
|
Organization name |
VIB
|
Department |
Nucleomics Core
|
Street address |
Herestraat 49 Box 816
|
City |
Leuven |
ZIP/Postal code |
B-3000 |
Country |
Belgium |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE35696 |
SIN3A-regulated LIF-responsive genes in MCF7 cells |
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