|
| Status |
Public on Jul 01, 2013 |
| Title |
CD3 lymphocytes from 12MO |
| Sample type |
genomic |
| |
|
| Source name |
CD3 lymphocytes isolated from peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD3 lymphocytes isolated from peripheral blood
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from whole bone marrow, whole peripheral blood or CD3 lymphocytes isolated from peripheral blood prior to treatment with lenalidomide, at the individual centers participating in the study. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer and agarose gel electrophoresis.
|
| Label |
biotin
|
| Label protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned according to the manufacturer's instructions.
|
| Scan protocol |
CEL files, generated by AGCC Viewer. CNCHP and LOHCHP, generated by Genotyping Console (all provided by Affymetrix)
|
| Description |
Hybridized to 6.0 Array
|
| Data processing |
CNCHP (from 6.0 arrays) provide data for copy number and LOH analysis.
Genotyping Console; algorithm-name = CN5
|
| |
|
| Submission date |
Feb 09, 2012 |
| Last update date |
Jul 01, 2013 |
| Contact name |
Mar Mallo |
| Organization name |
Josep Carreras Leukaemia Research Institute
|
| Street address |
Ctra de Can Ruti, s/n, camà de les escoles. Edifici IMPPC
|
| City |
Badalona |
| ZIP/Postal code |
08916 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE35656 |
Affymetrix SNP array data for myelodysplastic syndrome with 5q deletion treated with lenalidomide |
|
