 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 08, 2025 |
| Title |
γδ T cells, WT1 |
| Sample type |
SRA |
| |
|
| Source name |
spleen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer’s protocol.
The libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer’s instructions
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Library name: Sample 1
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Raw reads of fastq format were firstly processed using fastp and the low quality reads were removed to obtain the clean reads.
The clean reads were mapped to the reference genome using HISAT2. FPKM of each gene was calculated and the read counts of each gene were obtained by HTSeq-count
Assembly: GRCm39
Supplementary files format and content: Matrix table with raw gene counts for every gene and every sample
Supplementary files format and content: Matrix table with FPKM values for every gene and every sample
|
| |
|
| Submission date |
Jan 03, 2025 |
| Last update date |
Jan 08, 2025 |
| Contact name |
xianghong wang |
| E-mail(s) |
wangxh2023@stu2022.jnu.edu.cn
|
| Organization name |
Zhuhai People’s Hospital
|
| Street address |
79 Kangning Road, Xiangzhou District
|
| City |
zhuhai |
| State/province |
Please Select |
| ZIP/Postal code |
519000 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN46073073 |
| SRA |
SRX27243416 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
