|
| Status |
Public on Jan 26, 2012 |
| Title |
P0 - lis1ko/+ Mix 3 |
| Sample type |
RNA |
| |
|
| Source name |
mutant Lis1 ko/+
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brain
genotype: mutant Lis1 ko/+
age: P0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent (Invitrogen)
|
| Label |
biotin
|
| Label protocol |
manufacture suggested biotin-labeled IVT reaction. 10 µg of total RNA was used in a maximum volume of 10 µl
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridized in the presence of BSA to an Affymetrix GeneChip™ following manufacture instructions. Volume for fragmented cRNA samples was 40 µl.
|
| Scan protocol |
GeneChip® Scanner 3000 with 1 number of scans per array with 2x image, pixel value 3 um and wavelenght 570 nm
|
| Description |
embryo at P0, biological rep3
|
| Data processing |
Microarray data was pre-processed via the Robust Multichip Average (RMA) method, as implemented in the Bioconductor suite of programs (http://www.bioconductor.org/) implemented in the R statistical programming language. Analysis of differential expression was performed using bi-weight analysis.
|
| |
|
| Submission date |
Jan 26, 2012 |
| Last update date |
Jan 26, 2012 |
| Contact name |
Tiziano Pramparo |
| Organization name |
Travere
|
| Department |
R&D
|
| Street address |
3611 Valley Centre Drive
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92132 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE35366 |
Global developmental gene expression and pathway analysis of normal brain development and mouse models of human neuronal migration defects |
|
