|
| Status |
Public on Dec 05, 2024 |
| Title |
hfCas13d_Vec-2 |
| Sample type |
SRA |
| |
|
| Source name |
E0771
|
| Organism |
Mus |
| Characteristics |
cell line: E0771
cell type: Breast cancer
treatment: Vector
batch: 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by TRIZOL.
The RNA sequencing library was prepared using the NEBNext polyA mRNA isolation kit and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
Library name: 7_2
|
| Data processing |
FASTQ files were aligned to the mouse reference genome GRCm39 (version Ensembl 112) by STAR 2.7.10b . Reads mapped to each gene were counted using STAR --quantMode.
Assembly: GRCm39
Supplementary files format and content: tab-delimited text files include read counts for each Sample
|
| |
|
| Submission date |
Dec 05, 2024 |
| Last update date |
Dec 05, 2024 |
| Contact name |
Chuanpeng Dong |
| E-mail(s) |
chuanpeng.dong@yale.edu
|
| Organization name |
Yale University School of Medicine
|
| Department |
Genetics
|
| Street address |
850 West Campus Dr
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06516 |
| Country |
USA |
| |
|
| Platform ID |
GPL23787 |
| Series (1) |
| GSE269516 |
Multiplexed inhibition of immunosuppressive genes with Cas13d for on-demand combinatorial cancer immunotherapy |
|
| Relations |
| BioSample |
SAMN45194959 |
| SRA |
SRX26991620 |
