|
| Status |
Public on Feb 05, 2025 |
| Title |
wt_R1 |
| Sample type |
SRA |
| |
|
| Source name |
wing disc
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: wing disc
cell type: whole wing disc
genotype: 1151 x w1118
group: wt
|
| Treatment protocol |
wing disc were disected in PBS and and added to DNA/RNA protection Reagent (Part of the Monarch® RNA Miniprep Kit)
|
| Growth protocol |
samples where crossed and the offspring were maintained by 29 °C until they were wandering larve stage (about 5 days after egg lay)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the Monarch from NEB (T2010S)
The library was prepared by using the ThruPLEX DNA-Seq Kit (Rubicon) for illumina sequencing.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
| |
|
| Description |
not_used_for Analysis
|
| Data processing |
Raw data were quality checked with FastQC
trimmed using Trimmomatic 0.36
mapped against dm6 with STAR
reads were counted by QoRTs
differential expression analyses were performed with standard conditions for DESeq2
Assembly: dm6
Supplementary files format and content: tab-delimited text file with raw counts
|
| |
|
| Submission date |
Nov 12, 2024 |
| Last update date |
Feb 05, 2025 |
| Contact name |
Katrin Domsch |
| E-mail(s) |
katrin.domsch@cos.uni-heidelberg.de
|
| Phone |
+49-6221-545519
|
| Organization name |
Heidelberg University
|
| Department |
COS - Developmental Biology
|
| Lab |
Ingrid Lohmann
|
| Street address |
INF 230
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL22106 |
| Series (1) |
| GSE281653 |
Autonomous function of Antennapedia (Antp) in adult muscle precursors directly connects Hox genes to adult muscle development |
|
| Relations |
| BioSample |
SAMN44694578 |
| SRA |
SRX26692186 |
