|
| Status |
Public on Jan 17, 2012 |
| Title |
2737_G3_OHT |
| Sample type |
genomic |
| |
|
| Source name |
Atm-/-TERT-ER_Tel_G3_OHT
|
| Organism |
Mus musculus |
| Characteristics |
recipient strain: SCID
genotype/variation: Atm-/-TERT-ER
telomere maintanence mechanism: Telomerase
generations: G3
treatment: 4OHT
|
| Treatment protocol |
Various generations of Atm-/-TERT-ER mice treated with 4-OHT or vehicle. Recipient SCID mice injected with G3 Atm-/-TERT-ER T-cell lymphomas were maintained either on 4-OHT or vehicle
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total genomic DNA were extracted with Qiagen DNeasy Blood & Tissue Kit following according to the manufacturer's instructions.
|
| Label |
Cy3-dUTP
|
| Label protocol |
The fragmented product is labeled via exo-Klenow with Cyanine 3-dUTP.
|
| |
|
| Hybridization protocol |
Hybridization was performed with the Tecan HS Pro hybridization station.
|
| Scan protocol |
Scanning was performed with Agilent scanner equipped Agilent Scanner Control software v7.0.
|
| Data processing |
Data were processed with the Feature Extraction (FE) software v9.5, normalized using LOcally-WEighted regression Scatterplot Smoother.
|
| |
|
| Submission date |
Jan 15, 2012 |
| Last update date |
Jan 17, 2012 |
| Contact name |
Jian Hu |
| Organization name |
MD Anderson Cancer Center
|
| Department |
Cancer Biology
|
| Lab |
Ron DePinho
|
| Street address |
1515 Holcombe Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL15128 |
| Series (2) |
| GSE35045 |
Genomic copy number profiling of different generations of Atm-/-TERT-ER T-cell lymphomas and associated resistant tumors following telomerase inhibition |
| GSE35046 |
Anti-telomerase therapy provokes ALT and mitochondrial adaptive mechanisms |
|
