|
| Status |
Public on Feb 08, 2007 |
| Title |
7pm B;Muscles;NONE;C57/BL6 clock KO;38846 |
| Sample type |
RNA |
| |
|
| Source name |
7pm B;Muscles;NONE;C57/BL6 clock KO;38846
|
| Organism |
Mus musculus |
| Characteristics |
7pm B;Muscles;NONE;C57/BL6 clock KO;38846
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissues were collected from 5 Clock/Clock mutants at each timepoint except for 34 and 46 hours after the onset of DD, when tissues from 10 Clock/Clock mice were collected and run as independent replicates. Mice were sacrificed by cervical dislocation, and the optic nerves were severed in complete darkness; brain dissection was performed using illumination from an infrared viewer (FJW Industries, Palatine, IL). SCNs were dissected out, pooled at a density of 5 per tube in 100 ?l RNAlater (Ambion, Austin, TX), frozen on dry ice, and stored at –80?C until use.
|
| Label |
biotin
|
| Label protocol |
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures except for use of a custom 24-channel fluidics machine
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
circadian muscle_clock mutant_24hr_gcrma
|
| Data processing |
gcRMA
|
| |
|
| Submission date |
Dec 06, 2005 |
| Last update date |
Feb 08, 2007 |
| Contact name |
John R Walker |
| E-mail(s) |
jwalker@gnf.org
|
| Phone |
858-812-1636
|
| Organization name |
Genomics Institute of the Novartis Research Foundation
|
| Lab |
Genetics Core
|
| Street address |
10675 John Jay Hopkins
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL1073 |
| Series (2) |
| GSE3746 |
Circadian skeletal muscle_wt and Clock mutants |
| GSE3751 |
Circadian rhythm in clock mutants |
|
