|
| Status |
Public on Aug 03, 2012 |
| Title |
CD4+_4 |
| Sample type |
genomic |
| |
|
| Source name |
CD4+ T cells
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
tissue/cell type: CD4+ T cells
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted and purified from all samples using QIAmp DNA Micro Kit (QIAGEN, Germany) according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation540 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
anti-CD4 magnetic-activated cell sorting
|
| Data processing |
The raw data from GenomeStudio software (v2011.1) methylation module (v1.9.0) were imported into R v. 2.13 and analyzed with the Bioconductor lumi package. Color channel imbalance adjusted, background corrected, and quantile normalized M values followed by fitting a Gamma distribution.
|
| |
|
| Submission date |
Jan 12, 2012 |
| Last update date |
Aug 03, 2012 |
| Contact name |
Lovisa E Reinius |
| E-mail(s) |
lovisa.reinius@ki.se
|
| Organization name |
Karolinska Institutet
|
| Department |
Department of Biosciences and Nutrition
|
| Street address |
Novum, Halsovagen 7-9
|
| City |
Huddinge |
| ZIP/Postal code |
14157 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE35069 |
Differential DNA Methylation in Purified Human Blood Cells |
|
