|
| Status |
Public on Mar 19, 2012 |
| Title |
GFP, control, rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
Kc cells
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: Kc cells
knockdown: GFP control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Qiagen RNA extraction was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Background control for Sxl.RNAi.3 and Sxl.MSL2.RNAi.2
|
| Data processing |
Raw data are preprocessed with robust multiarray average (RMA) method.
|
| |
|
| Submission date |
Jan 04, 2012 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Joshua WK Ho |
| E-mail(s) |
jwho@rics.bwh.harvard.edu
|
| Organization name |
Brigham and Women's Hospital
|
| Department |
Department of Medicine
|
| Lab |
Division of Genetics
|
| Street address |
41 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE34858 |
Sequence-specific targeting of dosage compensation in Drosophila favors an active chromatin context (mRNA) |
| GSE34859 |
Sequence-specific targeting of dosage compensation in Drosophila favors an active chromatin context |
|
| Relations |
| Reanalyzed by |
GSE119084 |
