|
| Status |
Public on Jul 18, 2012 |
| Title |
CD4+ CD69-_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Thymus
|
| Organism |
Mus musculus |
| Characteristics |
hematopoiesis population: CD4+ CD69-
strain: C57BL/6
age: 2 months
gender: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip 3000 7G with GCOS version 1.4.0.036.
|
| Description |
p45_s178.cel
Sample name: 45_178_CD4+ CD69-
|
| Data processing |
The data were normalized using GC-RMA method on Gene Expression Commons system (http://gexc.stanford.edu/)
|
| |
|
| Submission date |
Dec 27, 2011 |
| Last update date |
Mar 11, 2014 |
| Contact name |
Jun Seita |
| E-mail(s) |
jseita@stanford.edu
|
| Phone |
650-725-5808
|
| Organization name |
Stanford University
|
| Department |
Institute for Stem Cell Biology and Regenerative Medicine
|
| Lab |
Weissman
|
| Street address |
Lokey Stem Cell Building #G3101, 265 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE34723 |
Gene Expression Commons: an open platform for absolute gene expression profiling |
|
