|
| Status |
Public on Dec 10, 2012 |
| Title |
C666 |
| Sample type |
RNA |
| |
|
| Source name |
NPC cell line
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: nasopharyngeal carcinoma
|
| Biomaterial provider |
School of Cancer Sciences, The University of Birmingham
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from biopsy samples using TRIzol Reagent (Invitrogen, USA) and purified by the RNeasy Mini Kit (Qiagen, UK).
|
| Label |
biotin
|
| Label protocol |
The RNA was subjected to three rounds of amplification followed by biotin labelling using an ExpressArt TR Nano amplification kit (AmpTec) and an Affymetrix IVT labelling kit.
|
| |
|
| Hybridization protocol |
Biotinylated RNA was fragmented and hybridised to Affymetrix Human Genome U133Plus2 Arrays according to the Affymetrix protocol.
|
| Scan protocol |
All arrays were washed and stained on an Affymetrix FS450 fluidics station then scanned using an Affymetrix GeneChip 3000 7G scanner as per Affymetrix procedures.
|
| Data processing |
Expression array data were analysed with GCOS using the default settings except that the target signal was set to 100.
|
| |
|
| Submission date |
Dec 19, 2011 |
| Last update date |
Dec 10, 2012 |
| Contact name |
Wenbin Wei |
| E-mail(s) |
Wenbin.Wei2@durham.ac.uk
|
| Organization name |
Durham University
|
| Department |
Biosciences
|
| Street address |
Stockton Road
|
| City |
Durham |
| ZIP/Postal code |
DH1 3LE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE34573 |
A Global View of the Oncogenic Landscape in Nasopharyngeal Carcinoma:An Integrated Analysis at the Genetic and Expression Levels |
|
