|
| Status |
Public on Jan 08, 2025 |
| Title |
PBMCs, Figure_1_RNA |
| Sample type |
SRA |
| |
|
| Source name |
Blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Blood
library type: mRNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10x Genomics protocol for Multiplexed samples with Feature Barcode technology for Protein using Barcode Oligo Capture (CG000673, Rev A).
PBMC enriched samples were processed according to the 10x Genomics protocol for Fixation of Cells and Nuclei (CG000478, Rev C). Probe hybridization was completed according to the 10x Genomics protocol for Multiplexed samples with Feature Barcode technology for Protein using Barcode Oligo Capture (CG000673, Rev A).
10x Genomics protocol for Multiplexed samples with Feature Barcode technology for Protein using Barcode Oligo Capture (CG000673, Rev A).
Chromium Fixed RNA Profiling for Multiplexed Samples (CG000527)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq X |
| |
|
| Description |
Hybridized Probe-based RNA capture on 10x Barcoded Beads Mulitplexed pool with samples: BL05111, BL05113, BL05402, PB05111, PB05113, PB05402
|
| Data processing |
10x Cell Ranger Pipeline
Scrublet Doublet Detection
Assembly: GRCh38
Supplementary files format and content: H5 cell by gene matrix, csv with doublet information, fcs files represent data for the flow cytometry results shown in our figure 2, fcs files represent data for the flow cytometry results shown in our figure 2
|
| |
|
| Submission date |
Jul 29, 2024 |
| Last update date |
Jan 08, 2025 |
| Contact name |
Allen Institute For Immunology |
| E-mail(s) |
xiaojun.li@alleninstitute.org
|
| Phone |
2065487135
|
| Organization name |
Allen Institute
|
| Street address |
615 Westlake Ave N, Seattle, WA
|
| City |
Seattle |
| State/province |
Washington |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL34281 |
| Series (1) |
| GSE273419 |
CyroSCAPE: Scalable Immune Profiling Using Cryopreserved Whole Blood for Multi-omic Single Cell and Functional Assays |
|
| Relations |
| BioSample |
SAMN41788226 |
| SRA |
SRX25490864 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM8428072_BL05111_2.csv.gz |
59.1 Kb |
(ftp)(http) |
CSV |
| GSM8428072_BL05111_sample_filtered_feature_bc_matrix_GEXcounts.csv.gz |
24.1 Mb |
(ftp)(http) |
CSV |
| GSM8428072_BL05113_2.csv.gz |
65.1 Kb |
(ftp)(http) |
CSV |
| GSM8428072_BL05113_sample_filtered_feature_bc_matrix_GEXcounts.csv.gz |
26.2 Mb |
(ftp)(http) |
CSV |
| GSM8428072_BL05402_2.csv.gz |
61.2 Kb |
(ftp)(http) |
CSV |
| GSM8428072_BL05402_sample_filtered_feature_bc_matrix_GEXcounts.csv.gz |
24.6 Mb |
(ftp)(http) |
CSV |
| GSM8428072_Fig_1_Final.rds.gz |
1001.7 Mb |
(ftp)(http) |
RDS |
| GSM8428072_Figure_1B.csv.gz |
344 b |
(ftp)(http) |
CSV |
| GSM8428072_Figure_1C.csv.gz |
522 b |
(ftp)(http) |
CSV |
| GSM8428072_Figure_1D.csv.gz |
17.9 Kb |
(ftp)(http) |
CSV |
| GSM8428072_Figure_1E.csv.gz |
14.7 Kb |
(ftp)(http) |
CSV |
| GSM8428072_PB05111_2.csv.gz |
43.7 Kb |
(ftp)(http) |
CSV |
| GSM8428072_PB05111_sample_filtered_feature_bc_matrix_GEXcounts.csv.gz |
17.3 Mb |
(ftp)(http) |
CSV |
| GSM8428072_PB05113_2.csv.gz |
66.4 Kb |
(ftp)(http) |
CSV |
| GSM8428072_PB05113_sample_filtered_feature_bc_matrix_GEXcounts.csv.gz |
24.9 Mb |
(ftp)(http) |
CSV |
| GSM8428072_PB05402_2.csv.gz |
62.8 Kb |
(ftp)(http) |
CSV |
| GSM8428072_PB05402_sample_filtered_feature_bc_matrix_GEXcounts.csv.gz |
25.6 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
