|
| Status |
Public on Dec 01, 2011 |
| Title |
sorted AML (CD34+CD38-) rep5 |
| Sample type |
RNA |
| |
|
| Source name |
sorted AML (CD34+CD38-)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: AML (CD34+CD38-)
|
| Treatment protocol |
Leukemic peripheral blood (PB) samples from five AML patients were collected after obtaining informed consent.Mononuclear cells (MNC) were purified from the samples by Ficoll– Hypaque density gradient centrifugation, resulting in approximately 1–3 108 cells per sample. To determine the CD34 and CD38 content, cells were labeled with anti-human CD34- fluorescein isothiocyanate (FITC) anti-CD38-phycoerythrin (PE) and IgG isotype control monoclonal antibodies (Miltenyi Biotec, Auburn, CA, USA) and the CD34 and CD38 expression levels were determined for each sample using FACS-Calibur (Becton Dickinson, Franklin Lakes, NJ, USA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 600 ng total RNA (Affymetrix wt_sensetarget_label_manual)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
affymetrix
|
| Data processing |
MAS5
|
| |
|
| Submission date |
Nov 30, 2011 |
| Last update date |
Oct 25, 2022 |
| Contact name |
jasmine Jacob |
| E-mail(s) |
j-jacob@sheba.health.gov.il
|
| Phone |
0523790500
|
| Organization name |
Sheba Medical Center at Tel HaShomer
|
| Street address |
haChevel 33
|
| City |
Shoham |
| State/province |
-Select- |
| ZIP/Postal code |
60850 |
| Country |
Israel |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE34044 |
Gene expression profiles of AML derived stem cells: similarity to hematopoietic stem cells |
|
