|
| Status |
Public on Jun 26, 2024 |
| Title |
BM, ASC, WT3 |
| Sample type |
SRA |
| |
|
| Source name |
Bone Marrow
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Bone Marrow
cell type: Antibody secreting cell (CD138+)
genotype: Wild type
treatment: SRBC Immunization
|
| Treatment protocol |
Mice were immunized with 100L PBS washed SRBC IP and tissues were harvesed at day 28.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were sorted into complete RPMI based on the markers listed in the sample metadata. Cells were spun down and then RNA was extracted using a Qiagen micro or mini kit.
Library construction was completed by the Yale Center for Genomic Analysis following a standard workflow.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
BMPC_Normalized_Counts.txt
15_234_343_S15
|
| Data processing |
Sequence reads were run using STAR, mapped to the reference genome (mm10), and read counts were extracted and normalized.
Assembly: mm10
Supplementary files format and content: Normalized counts txt files [counts per million (CPM)]
|
| |
|
| Submission date |
Jun 06, 2024 |
| Last update date |
Jun 26, 2024 |
| Contact name |
Anis Barmada |
| Organization name |
Yale University
|
| Department |
Department of Immunobiology
|
| Lab |
Carrie Lucas Lab
|
| Street address |
300 Cedar Street
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06519 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE269303 |
PI3Kg in B cells promotes antibody responses and generation of antibody-secreting cells [RNA-seq] |
|
| Relations |
| BioSample |
SAMN41720540 |
| SRA |
SRX24829147 |
