|
Status |
Public on May 28, 2024 |
Title |
HD (HDVax treated CD4+ T cells from T3 TILs) FBC |
Sample type |
SRA |
|
|
Source name |
Tumor
|
Organism |
Mus musculus |
Characteristics |
tissue: Tumor cell line: T3 Sarcoma cell type: Tumor antigen specific CD4 T cells genotype: WT mice treatment: vaccines containing high doses (15 ug) of mItgb1 SLP
|
Extracted molecule |
protein |
Extraction protocol |
Live sorted cells were processed by the Genome Institute at Washington University Library construction was generated using 10x libraries using 10X 5'v2 Single Cell RNAseq
|
|
|
Library strategy |
RNA-Seq |
Library source |
other |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
10x Data was processed with the Cell Ranger pipeline, version 6.1.1. The downstream analysis was performed using the Seurat package. Assembly: mm10 Supplementary files format and content: Comma separated files and matrix files
|
|
|
Submission date |
May 24, 2024 |
Last update date |
May 28, 2024 |
Contact name |
Vladimir Sukhov |
E-mail(s) |
sukhov@wustl.edu
|
Organization name |
Washington University in St. Louis
|
Department |
Immunology
|
Street address |
660. S Euclid Ave.
|
City |
St. Louis |
ZIP/Postal code |
63110 |
Country |
USA |
|
|
Platform ID |
GPL24247 |
Series (2) |
GSE268301 |
Single-cell RNA sequencing of antigen specific CD4+ T cells induced by high vs low doses of MHC-II restricted neoantigens |
GSE268302 |
RNA sequencing of antigen specific CD4+ T cells induced by high vs low doses of MHC-II restricted neoantigens |
|
Relations |
BioSample |
SAMN41524108 |
SRA |
SRX24684153 |