|
| Status |
Public on May 03, 2006 |
| Title |
puroD replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
c17.2 mouse neural stem cell line
|
| Organism |
Mus musculus |
| Characteristics |
pD is a clonal control (puromycin resitant) stably-expressing stem cell line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA probes were labeled according to the supplier’s instructions (Affymetrix, Santa Clara, CA, USA). First strand synthesis was carried out by a T7-(dT)24 primer and SuperScript II Reverse Transcriptase (Gibco BRL Life Technologies) using 10?g of total RNA sample (Qiagen) from c17.2 neural stem cells stably transfected with the orphan nuclear receptor Nurr1 (clone42 and clone 48) or stably transfected with empty puromycin control vector (puro B and puroD)
|
| Label |
biotin
|
| Label protocol |
Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures, no PolyA controls used
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
puroD is a clonal control(puromycin resistant) stable c17.2 neural stem cell line with similar proliferation rate as clone 48 and therefore both were matched for subsequent analysis.
|
| Data processing |
Affymetrix Microarray Suite version 5.0
|
| |
|
| Submission date |
Nov 04, 2005 |
| Last update date |
Nov 07, 2005 |
| Contact name |
Kyle Matthew Sousa |
| Phone |
+46 8 5248 7866
|
| Fax |
+46 8 34 1960
|
| URL |
http://www.cajal.mbb.ki.se
|
| Organization name |
Molecular Neurobiology
|
| Department |
Medical Biochemistry and Biophysics
|
| Lab |
Karolinska Institute
|
| Street address |
Scheeles väg 1 A1 Plan 2
|
| City |
Stockholm |
| ZIP/Postal code |
17177 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE3571 |
Comparison of neural stem cell lines overexpressing Nurr1 to clonal controls |
|
