|
| Status |
Public on Jul 16, 2024 |
| Title |
B19238 : F : f/f : f/+ : -/- : + |
| Sample type |
RNA |
| |
|
| Source name |
liver of HBV transgenic mice
|
| Organism |
Mus musculus |
| Characteristics |
Sex: F
host foxa1 genotype: f/f
host foxa2 genotype: f/+
host foxa3 genotype: -/-
host albcre: +
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from liver of HBV transgenic mice as described (Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162: 156–159. doi:10.1006/abio.1987.9999)
|
| Label |
n/a
|
| Label protocol |
n/a
|
| |
|
| Hybridization protocol |
Hybridization of liver RNA to a specifically designed code set as per the manufacturers instructions.
|
| Scan protocol |
NanoString nCounter gene expression transcript counting was used to quantify the levels of a panel of genes using a specifically designed code set as per the manufacturers instructions.
|
| Data processing |
Data were quality controlled and normalized using the nSolver Analysis Software 4.0 (NanoString Technologies). RNA expression levels were normalized to mouse Cnot1, Rps29, Slc9a8, Mrps5 and Scarb1 RNA controls.
|
| |
|
| Submission date |
Apr 03, 2024 |
| Last update date |
Jul 16, 2024 |
| Contact name |
Mark Maienschein-Cline |
| E-mail(s) |
mmaiensc@uic.edu
|
| Organization name |
University of Illinois at Chicago
|
| Department |
Research Resources Center
|
| Lab |
Center for Research Informatics
|
| Street address |
1819 W Polk Ave, Rm 336 M/C 789
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60612 |
| Country |
USA |
| |
|
| Platform ID |
GPL34356 |
| Series (2) |
| GSE263120 |
Foxa-deficiency Restricts Hepatitis B Virus Biosynthesis Through Epigenic Silencing [NanoSting] |
| GSE263121 |
Foxa-deficiency Restricts Hepatitis B Virus Biosynthesis Through Epigenic Silencing |
|
