ATCC cell line <a href="http://www.atcc.org/SearchCatalogs/longview.cfm?view=ce,4949223,HTB-38&text=HTB%2D38">HTB-38</a>. Colorectal adenocarcinoma in a 44 year old female. Ultrastructural features include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough ER with free ribosomes, lipid droplets, few primary and many secondary lysosomes. No virus particles. HT-29 cells express urokinase receptors, but do not have detectable plasminogen activator activity. There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. The p53 antigen is overproduced. The cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV). The line is positive for expression of c-myc, K-ras, H-ras, N-ras. Myb, sis and fos oncogenes. N-myc oncogene expression was not detected. The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-;?) with a deleted short arm), t(7q;?), t(10q;?), i(13q), 19q+a; M6, ?t(8q;9q-), ?Xp, M9, 6q+, t(13;?)a, t(13;?)b, 19q+b, M14, M15, 15p+, and Xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis.
