|
| Status |
Public on Jan 27, 2024 |
| Title |
Muscle_SMAD4_wt_replicate1 |
| Sample type |
SRA |
| |
|
| Source name |
Mouse Muscle
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Mouse Muscle
cell line: Skeletal Muscle Satellite Cells
genotype: Smad4 f/+:Pax7 CreER/CreER:R26 YFP/YFP
treatment: Cardiotoxin treatment and Collagenase digestion of tissue
|
| Treatment protocol |
Treated with cardiotoxin
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
40 h after the CTX injury, MuSCs were isolated from the digested gaskin muscles using FACS and then underwent RNA extraction using the NucleoSpin RNA XS kit from Macherey-Nagel
Smart Seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Read alignment with STAR v2.7.5c
Feature counts and creation of count matrix using Cufflinks v2.2.2, downstream anlysis using DESEQ2
Assembly: mm10
Supplementary files format and content: Tab-separated FPKM-normalized gene counts for each sample
|
| |
|
| Submission date |
Jan 26, 2024 |
| Last update date |
Jan 30, 2024 |
| Contact name |
Yarui Diao |
| E-mail(s) |
yarui.diao@duke.edu
|
| Phone |
9196847090
|
| Organization name |
Duke University School of Medicine
|
| Department |
Cell Biology
|
| Lab |
Diao Lab
|
| Street address |
307 Research Drive, Box 3709
|
| City |
Durham |
| State/province |
North Carolina |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE199499 |
Single-cell chromatin accessibility profiling reveals a self-renewing muscle satellite cell state |
|
| Relations |
| BioSample |
SAMN39621539 |
| SRA |
SRX23409670 |
