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Sample GSM803360 Query DataSets for GSM803360
Status Public on Sep 29, 2011
Title HudsonAlpha_ChipSeq_A549_Pol2_EtOH_0.02pct_PCR2x
Sample type SRA
 
Source name A549
Organism Homo sapiens
Characteristics datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell description: epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis
antibody antibodydescription: mouse monoclonal, clone 8WG16. Antibody Target: POL2
antibody targetdescription: RNA Polymerase II
antibody vendorname: Covance
antibody vendorid: MMS-126R
treatment description: 1 h with 0.02% Ethanol (Myers)
protocol description: a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)
controlid: SL307
labexpid: SL245,SL334
replicate: 1,2
softwareversion: MACS
antibody vendorname: Covance
antibody vendorid: MMS-126R
treatment: EtOH_0.02pct
treatment description: 1 h with 0.02% EtOH (Myers)
protocol: PCR2x
protocol description: 2-cycles of PCR (Myers)
controlid: SL307
labexpid: SL245
replicate: 1
replicate description: tier 1
Biomaterial provider ATCC
Treatment protocol EtOH_0.02pct
Growth protocol A549_protocol.pdf
Extracted molecule genomic DNA
Extraction protocol Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer
 
Data processing http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
 
Submission date Sep 28, 2011
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL9052
Series (1)
GSE32465 Transcription Factor Binding Sites by ChIP-seq from ENCODE/HAIB
Relations
SRA SRX100405
BioSample SAMN00738322
Named Annotation GSM803360_hg19_wgEncodeHaibTfbsA549Pol2Pcr2xEtoh02RawRep1.bigWig
Named Annotation GSM803360_hg19_wgEncodeHaibTfbsA549Pol2Pcr2xEtoh02RawRep2.bigWig

Supplementary file Size Download File type/resource
GSM803360_hg19_wgEncodeHaibTfbsA549Pol2Pcr2xEtoh02PkRep1.broadPeak.gz 567.3 Kb (ftp)(http) BROADPEAK
GSM803360_hg19_wgEncodeHaibTfbsA549Pol2Pcr2xEtoh02PkRep2.broadPeak.gz 787.4 Kb (ftp)(http) BROADPEAK
GSM803360_hg19_wgEncodeHaibTfbsA549Pol2Pcr2xEtoh02RawRep1.bigWig 56.4 Mb (ftp)(http) BIGWIG
GSM803360_hg19_wgEncodeHaibTfbsA549Pol2Pcr2xEtoh02RawRep2.bigWig 69.6 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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