|
Status |
Public on Sep 29, 2011 |
Title |
HudsonAlpha_ChipSeq_A549_Pol2_EtOH_0.02pct_PCR2x |
Sample type |
SRA |
|
|
Source name |
A549
|
Organism |
Homo sapiens |
Characteristics |
datatype: ChipSeq datatype description: Chromatin IP Sequencing cell description: epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis antibody antibodydescription: mouse monoclonal, clone 8WG16. Antibody Target: POL2 antibody targetdescription: RNA Polymerase II antibody vendorname: Covance antibody vendorid: MMS-126R treatment description: 1 h with 0.02% Ethanol (Myers) protocol description: a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers) controlid: SL307 labexpid: SL245,SL334 replicate: 1,2 softwareversion: MACS antibody vendorname: Covance antibody vendorid: MMS-126R treatment: EtOH_0.02pct treatment description: 1 h with 0.02% EtOH (Myers) protocol: PCR2x protocol description: 2-cycles of PCR (Myers) controlid: SL307 labexpid: SL245 replicate: 1 replicate description: tier 1
|
Biomaterial provider |
ATCC
|
Treatment protocol |
EtOH_0.02pct
|
Growth protocol |
A549_protocol.pdf
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina Genome Analyzer |
|
|
Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
|
|
|
Submission date |
Sep 28, 2011 |
Last update date |
May 15, 2019 |
Contact name |
ENCODE DCC |
E-mail(s) |
encode-help@lists.stanford.edu
|
Organization name |
ENCODE DCC
|
Street address |
300 Pasteur Dr
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305-5120 |
Country |
USA |
|
|
Platform ID |
GPL9052 |
Series (1) |
GSE32465 |
Transcription Factor Binding Sites by ChIP-seq from ENCODE/HAIB |
|
Relations |
SRA |
SRX100405 |
BioSample |
SAMN00738322 |
Named Annotation |
GSM803360_hg19_wgEncodeHaibTfbsA549Pol2Pcr2xEtoh02RawRep1.bigWig |
Named Annotation |
GSM803360_hg19_wgEncodeHaibTfbsA549Pol2Pcr2xEtoh02RawRep2.bigWig |