|
| Status |
Public on Jul 01, 2024 |
| Title |
1038-G-DHM |
| Sample type |
SRA |
| |
|
| Source name |
small intestine
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: small intestine
condition: Growth
treatment: Donor Human Milk
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was harvested using RNEasy Mini Plus Kit (Qiagen). 100ug of RNA was used for library preparation.
RNA libraries were prepared utilizing the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Human genome assembly GRCh38 was used for reads alignment with STAR package
Counts tables were generated with featureCounts.
Assembly: GRCh38.110
Supplementary files format and content: comma-separated values (CSV) with raw counts for each sample.
|
| |
|
| Submission date |
Jan 17, 2024 |
| Last update date |
Jul 01, 2024 |
| Contact name |
Eduardo Gabriel Gonzalez Santiago |
| E-mail(s) |
eduardo.gonzalezsantiago@yale.edu
|
| Phone |
7874090472
|
| Organization name |
Yale University
|
| Department |
Pediatrics
|
| Lab |
Konnikova Lab
|
| Street address |
375 Congress Ave Rm 407
|
| City |
New Haven |
| State/province |
Connnecticut |
| ZIP/Postal code |
06519 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE253501 |
Maternal and donor human milk support robust intestinal epithelial growth and differentiation in a fetal intestinal organoid model |
|
| Relations |
| BioSample |
SAMN39475921 |
| SRA |
SRX23265536 |
