|
| Status |
Public on Aug 31, 2012 |
| Title |
Albumin-Cre E2f7loxp/loxp E2f8loxp/loxp liver 2m 78KO 4 (Affymetrix) |
| Sample type |
RNA |
| |
|
| Source name |
liver 2 months
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
age: 2 months
genotype: Albumin-Cre E2f7loxp/loxp E2f8loxp/loxp
genetic background: FVB
|
| Treatment protocol |
Adult liver tissues were collected at described time.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse 430.2 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Gene Chips were scanned using the Affy GeneChip Scanner 3000.
|
| Description |
liver 2 months gene expression
|
| Data processing |
The data were analyzed with RMAExpress (version 1.0 beta 4) and normalized using Quantile Normalization. Robust mean analysis (RMA) was performed with default parameter settings and the output was log transformed.
|
| |
|
| Submission date |
Sep 23, 2011 |
| Last update date |
Sep 01, 2012 |
| Contact name |
Madhu Ouseph |
| E-mail(s) |
madhu.ouseph@osumc.edu
|
| Phone |
6142922459
|
| Organization name |
The Ohio State University
|
| Street address |
460w 12th Ave
|
| City |
Columbus |
| State/province |
Ohio |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE32354 |
Expression data from E2f7/E2f8 and E2f1/E2f2/E2f3 null liver (Affymetrix) |
| GSE32355 |
E2f7/E2f8 and E2f1/E2f2/E2f3 null and wild type liver along with E2f7/E2f8 null and wild type trophoblast giant cells |
|
