|
| Status |
Public on Nov 30, 2011 |
| Title |
pre-B_3h_b-Est_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
B to M transdifferentiation sample
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Bone marrow derived cells (pre-B)
infection: C/EBPaER-GFP retrovirus
agent: b-Est
time: 3h
|
| Treatment protocol |
GFP pos. cells were sorted and afetr re-seeding on stroma induced with b-Est. GFP pos cell were sorted at different imes thereafter
|
| Growth protocol |
Pre-B cells were isolated, placed in duplicate on stromal cells plus cytokines, infected with an C/EBPaER-GFP retrovirus, GFP pos. cells sorted and after re-seeding on stroma induced with b-Est. Green cells were sorted at different times
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated by Trizol
|
| Label |
Biotin
|
| Label protocol |
RNAs were processed for Affymetrix 430.2 arrays as described in the manufacturer's instructions
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Gene expression data from B to M transdifferentiation
2391_aER_3h_bEST_2.CEL
|
| Data processing |
The data have been normalized with RMA method, using Bioconductor in R
|
| |
|
| Submission date |
Sep 23, 2011 |
| Last update date |
Nov 30, 2011 |
| Contact name |
Thien-Phong Vu Manh |
| Organization name |
CIML
|
| Street address |
163 avenue de Luminy
|
| City |
marseille |
| ZIP/Postal code |
13288 |
| Country |
France |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE32330 |
Expression data from C/EBP alpha induced transdifferentiation of pre-B cells into macrophages |
|
